Friday night I did a miniprep, using a dremelfuge with 3000 Rotations per minute. I did three samples.

Today I checked the DNA content of the probes in my universities lab, and it was 209 ng/uL , 176 and 52. With the last one I took only half the ingredients, because it was written  in the protocol. But I took 3ml overnight culture instead of 1.5mL so I ‘knew’ I had to double the ingredients.

The first two are quite usable in any purpose such as restriction & making new recombinant plasmids. The third sample is still useful for transformation.

Advertisements