Hi,

I heard that some microbiologists for routine plasmid amplification just put a colony of E.Coli into LB in an Eppi, add plasmid, incubate it  for 30 minutes and then place on selective medium. Transformation efficiency is very bad, so not good for ligation!

Had to try that out today… Let’s see what will happen 😀

So I did three tries:

One in H2O with 0,9% NaCl 37°C,    LB 37°C     and one in LB 20°C…

Just inocculated E.Coli with a pipette tip into a sterile eppendorf tube, with 200 uL medium/water. Added a GFP-coding plasmid… Waited for 30 Minutes and plated them on LB-Apmicillin-Agar…
Tomorrow we’ll see if colonies were formed and if they glow under UV-Light also.

Lab Strain

Eppis

As a result, these plates were obtained…

SAMSUNG

With a bacterial lawn growing on the plates… Seems the Ampicillin of those (months old) plates has decayed too much… Guess there will be a chance to do it again soon…

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